MOROCCAN SOCIETY OF BIOCHEMISTRY AND MOLECULAR BIOLOGY (SMBBM)
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INTERNATIONAL UNION OF BIOCHEMISTRY AND MOLECULAR BIOLOGY (IUBMB)
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FEDERATION OF THE AFRICAN SOCIETIES OF BIOCHEMISTRY AND MOLECULAR BIOLOGY (FASBMB)
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Cadi Ayyad University 30th anniversary
UCAM

FACULTE DES SCIENCES-SEMLALIA, MARRAKECH (FSSM)
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PREVIOUS MANIFESTATIONS
CONGRESS 2002 (CASABLANCA)
CONGRESS 2004 (MARRAKECH)
CONGRESS 2006 (AGADIR)

 

 

 

THIRD SMBBM INTERNATIONAL CONGRESS OF BIOCHEMISTRY,
IUBMB SPECIAL MEETING ON PLANT STRESSES & 6 th. CONGRESS OF FASBMB. A JOINT EVENT IN MARRAKECH (MOROCCO), APRIL 20-25, 2009

Press release (arabic)
Press release (French)
Article release (English)

SPEAKERS CV

Prof. Juan J. CALVETE

Full Professor; Head of the Structural Proteomics Laboratory. Instituto de Biomedicina de Valencia, C.S.I.C., Jaime Roig 11,
46010-Valencia (Spain). Phone: +34 96 339 1778; Fax: +34 96 369 0800; Email: jcalvete@ibv.csic.es

Lecture Title: 'Snake venomics and antivenomics: from ophidian evolution to biomedicine'

1998- Staff Researcher at Researcher at Instituto de Biomedicina,
CSIC (Spain) (Structural Proteomics)
2001- Professor at Universidad de la República (Uruguay)
(Biology)

1993-98: Staff Researcher at Veterinary Highschool, Hannover (Ger) (Structural Biology)
1998- Staff Researcher at Researcher at Instituto de Biomedicina, CSIC (Spain) (Structural Proteomics)
2001- Professor at Universidad de la República (Uruguay) (Biology)

PROFESSIONAL POSITIONS
Since 1989 Established Investigator of the Spanish Research Council (CSIC)
1993-1998 Staff Researcher of the Veterinary High School of Hannover, Germany

AWARDS
1989 Young Scientist Award of the International Society on Thrombosis and Haemostasis

PUBLICATIONS (Selected publications from the last 5 years, out of 260 papers in peer-reviewed journals and book chapters since 1986)

Obtustatin, a potent and selective inhibitor of 1 1 integrin in vitro and angiogenesis in vivo
Marcinkiewicz, C., Weinreb, P.H., Calvete, J.J., Kisiel, D.G., Mousa, S.A., Tuszynski, G.P. & Lobb, R.R.Cancer Res. 63 (2003) 2020-2023

NMR solution structure of the non-RGD disintegrin obtustatin. Moreno-Murciano, M.P., Monleón, D., Marcinkiewicz, C., Calvete, J.J. & Celda, B. J. Mol. Biol. 329 (2003) 135-145

Snake venom disintegrins. Novel dimeric disintegrins and structural diversification by disulphide bond engineering. Calvete, J.J., Moreno-Murciano, M.P., Theakston, R.D.G., Kisiel, D.G. & Marcinkiewicz, C. Biochem. J. 372 (2003) 725-734

Concerted motions of the integrin-binding loop and the C-terminal tail of the non-RGD disintegrin obtustatin. Monleón, D., Moreno-Murciano, M.P., Kovacs, H., Marcinkiewicz, C., Calvete, J.J. & Celda, B. J. Biol. Chem. 278 (2003) 45570-45576

Snake venomics: characterization of protein families in Sistrurus barbouri venom by cysteine mapping, N-terminal sequencing, and MS/MS analysis. Juárez, P., Sanz,L. & Calvete, J.J. Proteomics 4 (2004) 327-338

Structural requirements of MLD-containing disintegrins for distinct inhibition of 4 and 9 integrins
Bazan-Socha, S., Kisiel, D.G., Young, B., Theakston, R.D.G., Calvete, J.J., Sheppard, D. & Marcinkiewicz, C. Biochemistry 43 (2004) 1639-1647

Conformation and concerted dynamics of the integrin binding site and the C-terminal region of echistatin revealed by homonuclear NMR. Monleón, D., Esteve, V., Kovacs, H., Calvete, J.J. & Celda, B. Biochem. J. 387 (2005) 57-66

Structure-function correlations of snake venom disintegrins. Calvete, J.J. Curr. Pharm. Design 11 (2005) 829-835

Snake venomics: comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina. Bazaa, A., Marrakchi, N., El Ayeb, M., Sanz, L. & Calvete, J.J.Proteomics 5 (2005) 4223-4235

The first crystal structure of a Mimosoideae lectin reveals a novel quaternary arrangement of a widespread domain. Gallego del Sol, F., Nagano, C.S., Cavada, B.S. & Calvete, J.J.
J. Mol. Biol. 353 (2005) 574-583

cDNA cloning and functional expression of jerdostatin, a novel RTS-disintegrin from Trimeresurus jerdonii and a specific inhibitor of the 1 1 integrin
Sanz, L., Chen, R-Q., Pérez, A., Hilario, R., Juárez, P., Marcinkiewicz, C., Monleón, D., Celda, B., Xiong, Y-L., Pérez-Payá, E. & Calvete, J.J., J. Biol. Chem. 280 (2005) 40714-40722

Conformational diversity of the Goodpasture antigen, the noncollagenous-1 domain of the 3 chain of collagen IV. Calvete, J.J., Revert, F., Blanco, M., Cervera, J., Tárrega, C., Sanz, L., Revert-Ros, F., Granero, F., Pérez-Payá, E., Hudson, B.G. & Saus, J.
Proteomics 6 (2006) S237-S244 (Proteomics 8 (2007) 217)

Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries. Insight into the evolution of the snake venom´s integrin inhibition system. Sanz, L., Bazaa, A., Marrakchi, N., Pérez, A., Chenik, M., Bel Lasfer, Z., El Ayeb, M. & Calvete, J.J., Biochem. J. 395 (2006) 385-392

Characterizing the tick carboxypeptidase inhibitor: Molecular basis for its two-domain nature
Arolas, J.L., Bronsoms, S., Ventura, S., Avilés, F.X. & Calvete, J.J., J. Biol. Chem. 281 (2006) 22906-22916

Venom proteomes of closely-related Sistrurus rattlesnakes with divergent diets
Sanz, L., Gibbs, H.L., Mackessy, S.P. & Calvete, J.J.J. Proteome Res. 5 (2006) 2098-2112

Snake venomics of Bitis gabonica gabonica. Protein family composition, subunit organization of venom toxins, and characterization of dimeric disintegrins bitisgabonin-1 and bitisgabonin-2
Calvete, J.J., Marcinkiewicz, C. & Sanz, L.
J. Proteome Res. 6 (2007) 326-336

KTS- and RTS-disintegrins: anti-angiogenic viper venom peptides specifically targeting the 1 1 integrin Calvete, J.J., Marcinkiewicz, C. & Sanz, L. Curr. Pharm. Des. 13 (2007) 2853-2859

Snake venomics. Protocols and applications. Calvete, J.J., Juárez, P. & Sanz, L.J. Mass Spectrom. 42 (2007) 1405-1414

Snake venomics of Bitis species reveals large intragenus venom toxin composition variation. Application to taxonomy of congeneric taxa. Calvete, J.J., Escolano, J. & Sanz, L.
J. Proteome Res. 6 (2007) 2732-2745

Insights into the structural basis of the pH-dependent dimer-tetramer equilibrium through crystallographic analysis of recombinant Diocleinae lectins. Nagano, C.S., Calvete, J.J., Barettino, D., Pérez, A., Cavada, B.S. & Sanz, L.
Biochem. J. 409 (2008) 417-428

Snake venomics of Central American pitvipers. Clues for rationalizing the distinct envenomation profiles of Atropoides nummifer and Atropoides picadoi. Angulo, Y., Escolano, J., Lomonte, B., Gutiérrez, J.M., Sanz, L. & Calvete, J.J. J. Proteome Res. 7 (2008) 708-719

Snake venomics of the South and Central American Bushmasters. Comparison of the toxin composition of Lachesis muta gathered from proteomic versus transcriptomic analysis
Sanz, L., Escolano, J., Ferretti, M., Biscoglio, M.J., Rivera, E., Crescenti, E.J., Angulo, Y., Lomonte, B., Gutiérrez, J.M. & Calvete, J.J. J. Proteomics 71 (2008) 46-60

CURRENT RESEARCH INTEREST
Structural Biology: Carbohydrate-binding proteins -spermadhesins (sperm surface-coating lectins involved in sperm physiology) and plant lectins; Non-conventional Proteomics; Snake venomics and antivenomics

Memberships in Professional Societies
Spanish Biophysical Society (1996-); Protein Society (1997-); American Association for Cell Biology (1997-); Biochemical Society (1999-); Spanish Society for Biochemistry and Molecular Biology (1999-); Spanish Proteomics Society (2004-); Spanish Society of Mass Spectrometry (2005-); International Society of Toxinology (2005-); American Society for Biochemistry and Molecular Biology (2006- ); Asociacion Española de Reproduccion Animal (2006- ); President of the Spanish Proteomics Society (http//:www.cbm.uam.es/seprot)

REVIEWER OF MANUSCRIPTS FOR:
Journal of Cell Biology, European Journal of Biochemistry/FEBS Journal, Biochemical Journal, Biological Chemistry Hoppe-Seyler, Andrologia, Journal of Experimental Animal Science, Reproduction in Domestic Animals, Toxicon, Fertilität, Thrombosis Research, Thrombosis and Haemostasis, FEBS Letters, Journal of Chromatography, Canadian Jounal of Physiology and Pharmacology , Electrophoresis, Fecundation, Acta Crystallographica Section D, Biotechnology and Applied Biochemistry, Journal of Biological Chemistry, Biochimica et Biophysica Acta, Archives of Biochemistry and Biophysics, Biophysical Journal, Structure, Proteomics, Protein Science, Journal of Physical Chemistry, Current Pharmaceutical Design, Biochemistry , Plant Molecular Biology, Journal of Thrombosis and Haemostasis, International Journal of Biological Macromolecules, Gene, Glycobiology, Genetics, Journal of Proteome Research, Journal of Pharmacy and Pharmacology, Comparative Biochemistry and Physiology, Cellular and Molecular Life Sciences, IUBMB Life, PROTEINS: Structure, Function & Bioinformatics, Molecular Reproduction and Development, Molecular Human Reproduction, Experimental Biology and Medicine, Planta Medica, Molecular and Cellular Proteomics, Journal of Mass Spectrometry, Journal of Experimental Biology, PROTEÓMICA-Revista de la Sociedad Española de Proteómica, Protein and Peptide Letters, Journal of Molecular Evolution, Carbohydrate Research, Biomacromolecules, PLoS ONE Medicine, BMC Genomics, Journal of Proteomics, Molecular Biology Reports

REVIEWER OF GRANT APPLICATIONS FOR:
Agencia Nacional de Evaluacion y Prospección, Ministerio de Ciencia y Tecnología, Programa de Proyectos Intramurales de Frontera 2006 del Consejo Superior de Investigaciones Científicas, Instituto de Salud Carlos III, Generalitat Valenciana, Fundación Séneca de la Agencia de Ciencia y Tecnología de la Región de Murcia, Organisation du Traite de L'Atlantique Nord (OTAN), International Foundation for Science (IFS, Stockholm), European Science Foundation (Strasbourg), Israel Science Foundation, Programa de Desarrollo Tecnológico del Ministerio de Educación y Cultura (Montevideo, Uruguay); International Polar Year o Netherlands Programme (IPYoNL) Netherlands Organisation for Scientific Research (NWO); Human Frontier Science Program Organization

MEMBER OF EDITORIAL BOARDS:
EDITORIAL ADVISER of BIOCHEMICAL JOURNAL (1999-)
EDITORIAL BOARD MEMBER of TOXICON (2006- )
EDITOR-IN-CHIEF OF JOURNAL OF PROTEOMICS (2007- )

Prof.Chidi Gideon Osuagwu

Department of Biomedical Technology
School of Health Technology, Federal University of Technology.
P.M.B. 1526, Owerri, Imo state, Nigeria
E-mail: chidi.osuagwu@gmail.com

Lecture Title: 'Oxidative stress; sicklecell syndrome as model'

EDUCATION:
1. PhD (Biochemistry, 1993) University of Port-Harcourt, Nigeria.
Dissertation on "Blood Redox Changes in Sickle Cell Disease"
2. Post-graduate studies in Medicinal Chemistry, University of Houston, Texas (1978-79).
3. MSc (Chemistry, 1978) Youngstown State University, Ohio.
4. BSc (Biology/Chemistry, 1975) Indiana University of Pennsylvania, Indiana Pennsylvania (Norah E Zink University Scholar, 1972-1975).
MEMBERSHIP OF PROFESSIONAL ASSOCIATIONS:
1. Fellow, Institute of Chartered Chemists of Nigeria (FICCON)
2. Member, Science Teachers Association of Nigeria (STAN)
3. Member, Nigerian Society of Biochemistry and Molecular Biology (NSBMB).
4. Member, Chemical Society of Nigeria (C.S.N).
5. Associate Member, Nigerian Institute for Biomedical Engineering (NIBES).
AREAS OF CURRENT RESEARCH INTEREST.
1. Investigation into the Bioenergetics/Redox aspects of Diseases; Sicklecell, Diabetes, Redox stress, Malaria, HIV?AIDS, etc.
2. Investigation into the relationship of structure and function in blood and other biological systems.
3. Studies on the chemistry of African traditional herbs/foods.
4. Investigation into the kinetic molecular origin of natural constants (Epsilon, Pi and Feigenbaum's number)
5. Studies on African Cosmology/indigenous Knowledge system in relation to Science.
PUBLICATIONS.
Have published sizable journal articles/books in Chemistry/Biochemistry/Systems' modeling, and African Cosmology.
SPECIAL RECOGNITION
1. Listed by Philosopedia (see Chidi Gideon Osuagwu at www.philosopedia.org). 2. Nomination to UNESCO in 2005 by the Imo State Government as a "Living Human Treasure" for work in African Cosmology (see www.unescong.org/Living Human Treasures).
JOB EXPERIENCE:
1. Currently, Senior Lecturer (Biochemistry), School of Health Technology, Federal University of Technology, Owerri, Nigeria. Have taught, since 1978, in different higher institutions in the United States and Nigeria.
CURRENT CIVIC/ADMINISTRATIVE RESPONSIBILITIES:
1. Member, Board of Directors, Alvana Community Bank (2001-date)
2. Member, Scientific Council, Whelan Research Academy, Owerri, Nigeria.
3. Head, Department of Biomedical Technology, Federal University of Technology, Owerri, Nigeria.

Dr. EL MOUALIJ Benaïssa

Scientific Director-Lecturer
Laboratory of Human Histology-CRPP, Institute of Pharmacy-CHU, Avenue de l'Hôpital, 1, Sart Tilman 4000, Liège, Belgium.Tel:00-32-4-366-43-28, Fax:00-32-4-366-43-21. E-mail: b.elmoualij@ulg.ac.be, Alternative:elmoualijb@hotmail.com
Sit web: http://www.ulg.ac.be/histohum

Lecture Title: 'Prion: a splendid and fatal molecular machine'

Current position: Since 2002 Research Scientist and Scientific Director at the Department of Human Histology- Centre of Research on Prion Protein "CRPP". University of Liège, Belgium.

EDUCATIONAL BACKGROUND:

1994-1999 Ph.D. in Biochemistry, University of Liège, Belgium.
1992-1994 Master. Sc. in Biochemistry, University of Liège, Belgium.
1987-1992 B. Sc. in Biochemistry, University Moulay Ismail of Méknès, Morocco.

EMPLOYMENT HISTORY:

2002- Present: Scientifc Director, Centre of Research on Prions Proteins, University of Liège
2000-2002 Research Scientist, CRPP, University of Liège
1999-2000 Postdoctoral affiliate, Center of protein engineering, University of Liège
1994-1999 Phd assistant-student, Laboratory of Bioenergetics, University of Liège.

PUBLICATIONS IN RELATION WITH THE CONFERENCES:

1 Ingrid Dupiereux, Willy Zorzi, Olivier Thellin, Olivier Pierard, Nicole Watt, NM Hooper, Ernst Heinen and Benaïssa ELMoualij. Potent protective effect of prion proteins on paraquat-induced mitochondrial uncoupling in Human Neuroblstoma SH-SY5Y cells. Journal of neurosciences research, 2008 86:653-659.

2 Dupiereux I, Zorzi W, Danièle Zorzi, Rachidi Walid, Bernard Lheureux, Heinen E, Elmoualij B. Study on the toxic mechanism of prion protein peptide 106-126 in neuronal and non neuronal cells J Neurosci Res. 2006 Aug 15;84(3):637-46.

3 Falisse-Poirrier N, Ruelle V, Elmoualij B, Zorzi D, Pierard O, Heinen E, De Pauw E, Zorzi W. Advances in immunoproteomics for serological characterization of microbial antigens. J Microbiol Methods. 2006 Dec;67(3):593-6. Epub 2006 Jul 5.

4 Dupiereux I, Zorzi W, Lins L, Brasseur R, Colson P, Heinen E, ElMoualij B.
Interaction of the 106-126 prion peptide with lipid membranes and potential implication for neurotoxicity. Biochem Biophys Res Commun. 2005 Jun 17; 331(4):894-901.

5 Stéphanie GOFFLOT, Manuel DEPREZ, Benaïssa EL MOUALIJ, Awad OSMAN, Jean-François THONNART, Olivier HOUGRAND, Ernst HEINEN, Willy ZORZI. Immuno-quantitative PCR for Prion Protein detection in Sporadic Creutzfeldt-Jakob Disease. Clinical chemistry. 2005 Sep;51(9):1605-1611.

6 Gofflot S, El Moualij B, Zorzi D, Melen L, Roels S, Quatpers D, Grassi J, Vanopdenbosch E, Heinen E, Zorzi W. Immuno-quantitative polymerase chain reaction for detection and quantitation of prion protein. J Immunoassay Immunochem. 2004;25(3):241-58.

7- Jean Barbeau, Benaïssa El Moualij, Ernst Heinen, Willy Zorzi . TRANSMISSION ET RéSISTANCE DES PRIONS : LA PRATIQUE DE LA MéDECINE DENTAIRE EN SERA T-ELLEAFFECTéE ? Journal de l'Ordre des dentistes du Québec Volume 43 Décembre 2006, 43(9) : 461-467.

PATENTS:

1- ElMoualij Benaïssa, Zorzi Willy, Zorzi Danièle, Heinen Ernst, Melen Laurence, Detection method by PCR, WO0131056, 2001-05-03, IqPCR using a chimeric DNA marker for amplification, WO0131056, 2001-05-03 ; EP1232283, 2002-08-21 ; DE60028233T, 2007-03-08.
2- ElMoualij Benaïssa, Zorzi Willy, Fabienne Eppaillard, Gilbert Legeay, Heinen Ernst. New supports for immunodetection and the storage or to the selective capture of molecules of interest, especially of prion proteins. WO2006105622, 2006-10-12

LIST OF MAIN RESEARCH GRANTS:

1- Development of an highly sensitive immuno-detection methods for prions proteins. 2000-2004.
2- Monitoring and elimination of the prions in the effluents and the environment of the slaughter-houses. 2003-2006.
3- Study of proteinopathies: from oxidative stress to therapeutics. 2004-2006.
4- Development of a "spin off, I-Detect" dedicated to proteinopathies. 2004-2006.
5- Neuroscreen: Developpement of a differential markers of Alzheimer, Parkinson and prions diseases. STREP project. 2006-2009.

MEMBER OF SCIENTIFIC SOCIETIES

Belgian Society of Biochemistry and Molecular Biology (SBB).
French society of biomaterials (BIOMAT).

Prof. Miguel A. De La Rosa

President-elect of the Spanish Society of Biochemistry and Molecular Biology (SEBBM) and Full Professor of Biochemistry and Molecular Biology at the University of Seville.

Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla y CSIC, Avda. Américo Vespucio, 49 41092-Sevilla, Spain
Tel: 34 954 489510, Fax: 34 954 460065. Email: marosa@us.es

Lecture Title: 'Protein Evolution and Geochemical Changes'

He is the founder and head of the Laboratory of Structural and Functional Proteomics, Institute of Plant Biochemistry and Photosynthesis, University of Seville and National Research Council (CSIC), Spain.

His major research interests are in the structure-function relationships of metalloproteins, protein evolution, photosynthesis and bioenergetics. He is author or co-author of 4 books, 2 patents, and over 140 original articles and book chapters, and has been invited speaker in more than 100 scientific meetings. He is/has been member of the FEBS Advanced Courses Committee (2003-06) and of the IUBMB Nominating Committee (2003-06), former President (2003-07) and Secretary General (1996-2001) of the Bioelectrochemical Society, editor of FEBS Letters (2005-), associate editor of Bioelectrochemistry (2005-), President of the 22nd IUBMB & 37th FEBS Congress (Seville, 2012).

Five Selected Publications (in the last few years):

Photosynthesis: A New Function for an Old Cytochrome?
F.P. Molina-Heredia, J. Wastl, J.A. Navarro, D. Bendall, M. Hervás, C. Howe & M.A. De la Rosa.
Nature (2003) 424, 33-34.

Electron Transfer between Membrane Complexes and Soluble Proteins in Photosynthesis (invited review).
M. Hervás, J.A. Navarro & M.A. De la Rosa.
Accounts of Chemical Research (2003) 36, 798-805.

Structure of the Complex between Plastocyanin and Cytochrome f from the Cyanobacterium Nostoc Sp. PCC 7119 as Determined by Paramagnetic NMR.
I. Díaz-Moreno, A. Díaz-Quintana, M.A. De la Rosa & M. Ubbink.
The Journal of Biological Chemistry (2005) 280, 18908-18915.

Thermal Unfolding of Plastocyanin from the Mesophilic Cyanobacterium Synechocystis sp. PCC 6803 and Comparison with its Thermophilic Counterpart from Phormidium laminosum.
M. Feio, A. Díaz-Quintana, J.A. Navarro & M.A. De la Rosa.
Biochemistry (2006) 45, 4900-4906.

A Proteomic Approach to Iron and Copper Homeostasis in Cyanobacteria (invited review).
B. De la Cerda, O. Catielli,R.V. Durán, J.A. Navaro, M. Hervás & M.A. De la Rosa.
Briefings in Functional Genomics and Proteomics (2008)6, 322-329.

Prof. Vincent Pryde Kehdingha TITANJI

Biotechnology Unit, University of Buea, Box 63, Buea, SW Province, Cameroon. E-mail: vpk.titanji@yahoo.com

Lecture title: 'Molecular cloning and characterization of UB 05 a dominant antigen of Plasmodium falciparum with immune protective potential'

Prof. Vincent P.K. Titanji obtained his PhD degree (1978) and 'Docent' title (1988) in Physiological Chemistry from Uppsala University and his MSc degree in Animal Biochemistry from Lomonosov Moscow State University (1973). Subsequently, he founded and headed the Biotechnology Center, Nkolbisson, University of Yaounde I (1986-1992). He was appointed to the University of Buea as Dean of the Faculty of Science (1993-1998) Deputy Vice-Chancellor for Teaching(1998-2005) and Vice Chancellor since 2006.

An expert in Protein Chemistry and Molecular Parasitology, he has devised diagnostic tests for river blindness, cloned vaccine candidates and identified new drug leads from medicinal plants for river blindness and malaria. He successfully trained 18 PhD graduates, 60 MSc students and has published widely in peer-reviewed journals.

Prof. Titanji was instrumental in the creation of the Cameroon Biochemical Society and has been President and Secretary General respectively of the Federation of African Societies of Biochemistry and Molecular Biology (FASBMB) and the Federation of African Immunological Societies (FAIS). A Knight of the Cameroon Order of Valour, Prof. Titanji is a Chartered Biologist (C. Biol), and Fellow (FI Biol) of the Institute of Biology, London; a Fellow of the Third World Academy of Sciences (FTWAS) and a recipient of the IFS/DANIDA Prize.

Matilde Barón

Department of Biochemistry, Cell and Molecular Biology of Plants. Estación Experimental del Zaidín. Profesor Albareda, 1. 18008 Granada, Spain

E-mail: matilde.baron@eez.csic.es

Lecture Title:"Proteomic and imaging analysis of pathogen-challenged plants"

Current position: Since 2006 Research Scientist at the Department of Biochemistry and Molecular Biology of Plants, Estación Experimental del Zaidín (EEZ), Spanish Council of Scientific Research (CSIC), Granada, Spain.

Graduated in Biological Sciences, with Special Award from the UGR. University of Granada (UGR, Spain) in 1978.
PhD in in Biological Sciences, with Special Award from the UGR. University of Granada. Spain 1984
Postdoctoral researcher at the Dept. of Plant Physiology and Plant Biochemistry University of Konstanz. Germany. 1985-1987.
Scientific Researcher. Department of Molecular Biology, Biochemistry and Cell Biology of Plants. EEZ. CSIC. 1988- 2006.

Research

Leader of the group Response of the chloroplast against biotic and abiotic stress.
Key words: photosynthesis, plant stress, plant pathogens, heavy metal toxicity, imaging techniques, chloroplast proteomics.
The group has a very dynamic national and international cooperation with countries as Morocco, Germany, Czech Republic, Hungary, Bulgaria, France. The group leader has obtained some international cooperation awards, as the Gabor Farkas one, from the Hungarian Scientia Amabilis Foundation.

Selected publications

M. L. Pérez-Bueno, J. Rahoutei, C. Sajnani, I. García- Luque and M. Barón.
Proteomic analysis of the oxygen-evolving-complex of Photosystem II under biotic stress. Studies on Nicotiana benthamiana infected with tobamoviruses. Proteomics, 4: 418-25, 2004.

Abdelmalek Hakmaoui, Matilde Barón, Mohammed Ater.
Environmental biotechnology. Screening Cu and Cd tolerance in Salix species from North Morocco. African. Journal of Biotechnology 5 (13), pp.1299-1302, 2006.

L. Chaerle, M. Pineda, D. Van Der Straeten, M. Barón
Robotized thermal and chlorophyll-fluorescence imaging of pepper mild mottle virus infection in Nicotiana benthamiana. Plant Cell Physiology. 47, 1323-1336, 2006.

M. L. Pérez-Bueno, M. Ciscato, M. vandeVen, I. García-Luque, R. Valcke, M. Barón. Imaging viral infection. Studies on Nicotiana benthamiana plants infected with the pepper mild mottle tobamovirus Photosynthesis Research, 90: 111-123, 2006.

Carlota Sajnani, Jorge L. Zurita, Mercedes Roncel, José M. Ortega, Matilde Barón, Jean-Marc Ducruet. Changes in photosynthetic metabolism induced by tobamovirus infection in Nicotiana benthamiana studied in vivo by chlorophyll thermoluminescence. New Phytologist 175:120-130, 2007.

Rodríguez-Moreno L., Pineda M., Soukupová J, Macho, A, Beuzón C.R., Barón M , Ramos C. Early detection of bean infection by pseudomonas syringae in asymptomatic leaf areas using chlorophyll fluorescence imaging. Phot. Res, 96:27-35, 2007. (

Mónica Pineda, László Gáspár, Fermín Morales, Zoltán Szigeti, Matilde Barón. Multi-colour fluorescence imaging: an useful tool to visualise compatible viral infection in plants (Photochemical Photobiology, 2008).

Selected projects as project leader

Supported from the Spanish Research Agencies
-Analisys of plant defence mechanisms against virus infection and supression of photosynthesis damage (BIO98-0860-CO2-02), CICYT, National Programme of Biotecnology. 1999/2001.
-Viral infections as biotic stress factor in photosynthesis. Metabolic changes and defense mechanism studies on infected leaves (BIO2001-1937-C02-02). MCYT, National Biotechnology Programme (Spain), 2001/2004.
-The chloroplast as target of the viral infection (BIO2004-04968-C02-02). MCyT. National Biotechnology Programme (Spain), 2004/2007.
- Evaluation of the impact of pathogen infection on the host plant by imaging and proteomic techniques . MEC (BIO2007-67874-C02-02), 2007-2008.

Dominique ROBY

Directeur de Recherches CNRS. Leader of the group " Resistance, susceptibility and cell death in Arabidopsis thaliana in response to pathogenic bacteria "
Institute : Laboratoire des Interactions Plantes/Microorganismes (LIPM), UMR INRA/CNRS 2594, Chemin de Borde Rouge, BP 52627, 31326 Castanet-Tolosan Cedex, France (http://www2.toulouse.inra.fr/centre/lipm). Tél. : 05 61 28 53 26, E-mail : dominique.roby@toulouse.inra.fr

Lecture Title: ' Key genes involved in resistance and hypersensitive cell death in Arabidopsis'

Director of the Federative Institute of Research " Agrobiosciences, Interactions and Biodiversity " of Toulouse
(http://www2.toulouse.inra.fr/ifr40/fr/1_Presentation/index.htm)

Education and Career History

Master of Plant Physiology, University Paul Sabatier, Toulouse, France, 1978.
PhD thesis in Plant Pathology, Université Paul Sabatier, France, 1981.
Doctorat d'Etat, Université Paul Sabatier, Toulouse, France, 1987.
Postdoctoral Research Associate (1987-1988), Department of Biological Sciences, Rutgers University, Newark, New-Jersey, USA
Postdoctoral Research Associate (1988-1989) E. DuPont de Nemours, Department of Agricultural Products, Experimental Station, Wilmington, Delaware, USA
Scientific Researcher CNRS Laboratoire de Physiologie Végétale, Toulouse, France (1982-1986) and Laboratory of Plant-Microorganism Interactions (LIPM) since 1986.

Representative publications

ROBY D., BROGLIE K., CRESSMAN R., BIDDLE P., CHET I. et BROGLIE R., 1990. Activation of a bean chitinase promoter in transgenic tobacco plants by phytopathogenic fungi. The Plant Cell 2, 999-1007.

GODARD F., LUMMERZHEIM M., SAINDRENAN P., BALAGUE C. and ROBY D. (2000). Hxc-2, an Arabidopsis mutant with altered hypersensitive response to Xanthomonas campestris pv. campestris. Plant J. 24(6), 749-761

PONTIER D., BALAGUE C., BEZOMBES-MARION I., TRONCHET M., DESLANDES L. and ROBY D. (2001). Identification of a novel pathogen-responsive element in the promoter of the tobacco gene HSR203J, a molecular marker of the hypersensitive response. Plant J. 26(5), 495-509.

VAILLEAU F., DANIEL X., TRONCHET M., MONTILLET JL., TRIANTAPHYLLIDES C. and ROBY D. (2002). A R2R3-MYB gene, AtMYB30, acts as a positive regulator of the hypersensitive cell death program in plants in response to pathogen attack. Proc. Natl. Acad. Sci. USA 99(15), 10179-10184..

BALAGUE C., LIN B., ALCON C., FLOTTES G., MALMSTROM S., KOHLER C., NEUHAUS G., PELLLETIER G., GAYMARD F. and ROBY D. (2003) HLM1, an essential component of signaling for the Hypersensitive Response, encodes a member of the CNGC ion channel family. The Plant Cell 15(2), 365- 379.

LORRAIN S., VAILLEAU F., BALAGUE C. and ROBY D. (2003) Lesion mimic mutants : keys for deciphering cell death and defense pathways in plants ? Trends in Plant Sci. 8(6), 263- 271.

LORRAIN S., LIN B., AURIAC M-C., KROJ, T., SAINDRENAN, P., NICOLE M., BALAGUE C. and ROBY D. (2004) VAD1, a novel GRAM domain-containing protein, is a regulator of cell death and defense responses in vascular tissues. The Plant Cell 16, 2217- 2232.

JOURNOT-CATALINO N., SOMSSICH I., ROBY D. and KROJ T. (2006) The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana. The Plant Cell 18, 1-14.

PERCHEPIED L., KROJ T., TRONCHET M., LOUDET O. and ROBY D. (2006). Natural variation in partial resistance to Pseudomonas syringae is controlled by two major QTLs in Arabidopsis thaliana. PloS ONE 1(1): e123. doi:10.1371/journal.pone 0000123.

RAFFAELE S., VAILLEAU F., LEGER A., JOUBES J., MIERSCHE O., HUARD C., BLEE E., MONGRAND S., DOMERGUE F. and ROBY D. (2008). A MYB Transcription Factor Regulates Very Long Chain Fatty Acid Biosynthesis for Activation of the Hypersensitive Cell Death in Arabidopsis. The Plant Cell (sous presse)

Technological transfer and recent contracts

Contrat de Collaboration avec les sociétés Vilmorin Clause & Cie, et Biogemma "Identification of new components of resistance/cell death pathways through the search of interacting partners of AtMYB30, a R2-R3 MYB transcriptional factor involved in the regulation of the hypersensitive response in Arabidopsis", 2003-2006, 198,906 euros.
Trilateral co-operation between Spain (McyT), France (Genoplante) and Germany (GABI) on Plant Genome Research "Diversity of disease resistance mechanisms in Arabidopsis DILEMA", Coordinators : D. Roby / J. Parker / C. Castresana, 2004-2007, 375 kE.
Contrat ANR Génoplante "Cyclic nucleotide-gated cation channels involved in hypersensitive cell death in Arabidopsis thaliana"(CHAT), 2006-2009.
Contrat ANR Génoplante "Identification of genes involved in Sclerotinia sclerotiorum resistance in Oilseed Rape and Sunflower"(Sclerotinia), 2006-2009.

Jesús V. JORRIN NOVO

"Titular" Professor at the University of Córdoba. Agricultural and Plant Biochemistry and Proteomics, Department of Biochemistry and Molecular Biology, Agricultural and Forestry High technical School (escuelta Técnica Superior de Ingenieros Agrónomos y de Montes)
Address: Campus de Rabanales, Edificio Severo Ochoa (C6), planta baja. 14071 Córdoba, Spain. Tel.: +34 957 218439; 957 218574, Fax: +34 957218439
E-mail: bf1jonoj@uco.es

Lecture Title: 'Proteomics in the study of plant responses to biotic and abiotic stresses'

AREAS OF EXPERTISE/INTEREST

Agricultural and Plant Biochemistry and Molecular Biology, Proteomics (plant proteomics, yeast proteomics), posttranslational modifications (redox proteome). Plant responses to biotic and abiotic stresses. Secondary metabolism (phenolics).

GENERAL

Head of the Agricultural and Plant Biochemistry and Proteomics, University of Córdoba
Secretary of the Dept. of Biochemistry and Molecular Biology, University of Córdoba (2004-2007)
Founder Member (2005) and Secretary (2005) of the Spanish Proteomics Sciety (SEProt)
Member of the Conference and Communication Committee of the European Proteomics Association (2006-)
Editor in Chief of "PROTEOMICA" (Journal of the Spanish SEProt
Executive Editor of the "Journal of Proteomics"
Member of the Editorial Board of "Proteomics" and "The Open Proteomics Journal"
Referee of: Proteomics, Phytochemistry, Physiologia Plantarum, Plant Science, Current proteomics, Journal of Plant Physiology, Weed Research, Journal of Heredity, Cell Research (China), ABB-Sinica,

FINNANCED RESEARCH PROJECTS (last 5 years, 2003-2008)

Application of proteomics to the study of sunflower, an strategic crop for Argentina and Spain (Spanish AECI, Ministry of Foreign Affair) (A/014378/07), 2007-2009

Plant proteomics in Europe" EUPP (European Plant Proteomics). EU. European Cooperation in the field of Scientific and Technical Research (COST) (OC-2006-217; FA 0603). 2006-2011.http://www.costfa0603.com/index.php.
Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae. TRANSLUCENT" (EU. P-E-01-06-17). 2007-2010. http://quiro.uab.es/Jarino/Translucent/
A comparative study of the redox proteome. Junta de Andalucía, Spain. 2007-2010.
The redox proteome: protein nitrosylation and glutathionylation as a regulating mechanism of the plant defense responses to pathogens. Spanish Ministry of Science and Education (DGI-MEC, Bio-2006.14790). 2006-2009.
Functional genomics (transcriptomics and proteomics) to study variability and responses to stresses in forest tree spp. (Quercus ilex, Quercus rubur, Pinus pinaster). Consejería de Medio Ambiente, Junta de Andalucía. 2005-2007.
Proteomics as a novel approach to study the Q. ilex decay disease. Monte de Piedad y Caja de ahorros de Huelva y Sevilla. 2004-2006.
New Strategies to Improve Grain Legumes for Food and Feed (EU, FP6-506223; WP 10, biotic stresses, Proteomic approach). 2004-2007. http://www.eugrainlegumes.org/summary/index.htm.

Resistance mechanism to parasitic angiosperms of the genus Orobanche. Spanish Ministry of Science and Education DGI, AGL2001-2420. 2003-2005.

Optimization of protocols for 2-DE análisis and its application to the proteome analysis of plant and yast (Andalusian Governement, University and Research Secretary). 2003-2004

Parasitic plant management in sustainable agriculture. EU COST Action 849, 2001-2006

1. Published papers 2004-2008 ( SCI Journals)

Sira Ehevarría-Zomeño, David Ariza, Inmaculada Jorge, Christof Lenz, Jesús Jorrín, Rafael M Navarro Cerrillo. 2008. Changes in the protein profile of Quercus ilex leaves in response to drought-stress and and recovery. Journal of Plant Physiology (in press)


VALLEDOR, L., CASTILLEJO, M.A., LENZ, C., RODRÍGUEZ, R., CAÑAL, M.J., JORRÍN, J. 2008. The 2-DE proteome of Pinus radiata needle tissue: analytical, biological variability and protein identification by LC/MS/MS and substitution-tolerant database searching. Journal of Proteome Research (in press).

CASTILLEJO M.A., MALDONADO A..M, OGUETA S., JORRÍN, J. 2008. Proteomic analysis of responses to drought stress in sunflower (Helianthus annuus) by two-dimensional gel electrophoresis and mass spectrometry. The Open Proteomics Journal (in press)

M.J. DUNN, C. GIL, C. KLEINHAMMER, F. LOTTSPEICH, S. PENNINGTON, J.-CH. SANCHEZ,J.P. ALBAR, L. BINI, F. CORRALES, G.L. CORTHALS, M.M. FOUNTOULAKIS, C. HOOGLAND, P. JAMES,O.N. JENSEN, C. JIMéNEZ, J. JORRÍN-NOVO, H.-J. KRAUS, H. MEYER, D. NOUKAKIS,P.M. PALAGI, D. PENQUE, A. QUINN, T. RABILLOUD. 2008. EuPA achieves visibility - An activity report on the first three years. Journal of Proteomics 1: 11-18.

E. PRATS, J.C. GARCIA GALINDO, M. E. BAZZALO, A. LEÓN, F. A. MACIAS, D. RUBIALES, J. V. JORRÍN. 2007. Antifungal activity of constitutive phenolic compounds from capitulum of a head rot-resistant sunflower genotype. : Journal of Chemcal Ecology, in press ( DOI 10.1007/s10886-007-9388-9)

JORRÍN, J., MALDONADO, A.M., CASTILEJO, MA. 2007. Plant proteome analysis: a 2006 update. Proteomics 7 2947 -2962

PéREZ-REINADO.E, RAMÍREZ-BOO.M, GARRIDO.J.J, JORRÍN.J.V AND MORENO.A. 2007. Toward global analysis of porcine alveolar macrophages proteins through two-dimensional electrophoresis and mass spectrometry. Developmental and Comparative Immunology 31: 1220-1232

E. PRATS, M. J. LLAMAS, J. JORRIN, AND D. RUBIALES. 2007. Constitutive Coumarin Accumulation on Sunfl ower Leaf Surface Prevents Rust Germ Tube Growth and Appressorium Diff erentiation. CROP SCIENCE,: 47: 1119-1124

RISPAIL.N, DITA M.-A, GONZÁLEZ-VERDEJO.C, PéREZ-DE-LUQUE.A, CASTILLEJO.M.-A, PRATS.E, ROMÁN.B, JORRÍN.J, RUBIALES.D. 2007. Plant resistance to parasitic plants: molecular approaches to an old foe. New Phytologist 173: 703-712.

FERNÁNDEZ-ACERO.F.J, JORGE.I, CALVO.E, VALLEJO.I, CARBÚ.M, CAMAFEITA.E, GARRIDO.C, LÓPEZ.J.A, JORRIN.J AND CANTORAL.J.M. 2007. Proteomic analysis of phytopathogenic fungus Botrytis cinerea as a potential tool for identifying pathogenicity factors, therapeutic targets and for basic research. Archives of Microbiology 187: 207-215.

ROLDÁN SERRANO.A, LUNA DEL CASTILLO.J, JORRÍN NOVO.J, FERNÁNDEZ OCAÑA.A, GÓMEZ RODRÍGUEZ.M.V. 2007. Chitinase and peroxidase activities in sunflower hypocotyls: effects of BTH and inoculation with Plasmopara halstedii. Biologia Plantarum 51: 149-152.

ECHEVARRÍA-ZOMEÑO, S., PéREZ-DE-LUQUE, A., JORRÍN, J., MALDONADO, A.M. 2006. Pre-haustorial resistance to broomrape (Orobanche cumana) in sunflower (Helianthus annuus): cytochemical studies. Journal of Experimental Botany, 57:4189-420

ROSSIGNOL.M, PELTIER.JB, MOCK.H.P, MATROS.A, MALDONADO.A, JORRÍN.J. 2006. Plant proteome analysis: a 2004-2006 update. Proteomics 6:5529-5548.

BÁRCENA JA, CALVETE JJ, MORENO-LOPEZ A, JORRÍN-NOVO JV. 2006. Editorial: out of Córdoba. Proteomics S1: 1-3-

JORGE I, NAVARRO, RM, LENZ C, ARIZA D, JORRÍN J. 2006. Variation in the holm oak leaf proteome at different plant developmental stages, between provenances and in response to drought stress. Proteomics S1´06: 207-214.

FERNÁNDEZ-ACERO FJ, JORGE I, CALVO E, VALLEJO I, CAMAFEITA LE, LÓPEZ JA, CANTORAL JM, JORRIN J. 2006. Two-dimensional electrophoresis protein profile of the phytopathogenic fungus Botrytis cinerea. Proteomics S1´06: 88-96.

CURTO, M., CAMAFEITA, E., LOPEZ, J.A., MALDONADO, A.M., RUBIALES, D., JORRÍN, J.V. 2006. A proteomic approach to study pea (Pisum sativum) responses to powdery mildew (Erysiphe pisi). Proteomics S1´06: 163-174.

JORRÍN, J.V., RUBIALES, D., DUMAS-GAUDOT, E., RECORBET, G., MALDONADO, A., CASTILLEJO, M.A., CURTO, M. 2006. Proteomics: a promising approach to study biotic stresses in legumes. A review. Euphytica 147: 37-47.

PRATS.E., BAZALO.M.E., JORRÍN.J.V. 2006. Fungitoxic effect of scopolin and related coumarins on Sclerotinia sclerotiorum. A way to overcome sunflower head rot. Euphytica 147: 451-460.

JORRÍN.J., BÁRCENA.J.A., CALVETE.J.J. 2005. Report on the First Congress of the Spanish Proteomics Society. Proteomics 5: 2712-2715.

PéREZ DE LUQUE.A., JORRÍN.J.V., CUBERO.J.I, RUBIALES, D. 2005. Orobanche crenata resistance and avoidance in pea (Pisum spp.) operate at different developmental stages of the parasite. Weed Science 45: 379-387.

JORGE, I., NAVARRO, M., LENZ, C., ARIZA, D., PORRAS, C., JORRÍN, J. 2005. . The Holm Oak leaf proteome. Analytical and biological variability in the protein expression level assessed by 2-DE and protein identification by MS/MS de novo sequencing and sequence similarity searching. Proteomics 5: 222-234.

CÁNOVAS.M, DUMAS-GAUDOT, E., RECORBET, G., JORRÍN, J., MOCK, H.P., ROSSIGNOL, M. 2004. Plant proteome analysis. Proteomics 4: 285-298.

CASTILLEJO, M.A., AMIOUR, N., DUMAS-GAUDOT, E., RUBIALES, D., JORRÍN, J.V. 2004. A proteomic approach to studying plant response to crenate broomrape (Orobanche crenata) in pea (Pisum sativum). Phytochemistry 65: 1817-1828.

Prof. Ramón Serrano

Instituto de Biología Molecular y Celular de Plantas (IBMCP), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.

Tel. 34-96-3877883; Fax 34-96-3877859
e-mail: rserrano@ibmcp.upv.es

Lecture Title: 'Probing basic cellular functions by abiotic stress'

Ramón Serrano is born at Valencia, Spain. He is Doctor Agricultural Enginneer, Research Professor of the spanish CSIC (Research Council) and Full Professor of Biochemistry and Molecular Biology at the Polytechnic University of Valencia, Spain.

Condecorated with the "Cruz de Alfonso X El Sabio" of the spanish government and Doctor Honoris Causa by the University of Regensburg, Germany.
The scientific background of Ramon Serrano is based on the training in the laboratories of three master scientists of modern biology: the spanish enzymologist Alberto Sols, the american bioenergeticist Efraim Racker and the american molecular biologists Gerald R. Fink. Their influence generated an interest at the molecular level on the connection between membrane transport, enzyme activity and cell growth.
The first achievement of Ramon Serrano as independent scientist was the characterization of the proton pump of yeast and plants. In animal cells, Jens C. Skou (Nobel Prize of Chemistry in 1997) discovered in the late fifties an ATPase which coupled ATP hydrolysis to Na and K transport across the plasma membrane. However, this enzyme does not exist in fungi and plants and the bioenergetics of the plasma membrane of non-animal cells was plunged into confusion for many years. Thanks to the work of Ramon Serrano in Spain, together with that of André Goffeau in Belgium and of Carolyn W. Slayman in USA, it became clear that the plasma membrane of fungi and plants is energized by a proton-pumping ATPase, not by a Na-K dependent enzyme.
Ramon Serrano was also at the frontline in the molecular cloning of the gene of the yeast proton pump, work performed in collaboration with Gerald R. Fink at the Whitehead Institute (MIT, Cambridge, Massachussetts, USA). Also, the plant proton pump was cloned for the first time in his laboratory at Heidelberg at the same time than in the laboratory of M.R. Sussman at Madison (USA). These non-animal H-ATPases belonged to the same protein family than the Na-K ATPase. Apparently, in the course of evolution, a primaeval pump had diverged into the animal and non-animal lineages. Animal cells have a primary sodium pump to regulate its volume and avoid swelling. On the other hand, fungal and plant cells obviate this problem by the presence of a rigid cell wall and exploit proton pumping.
Further work of Ramon Serrano defined the essential residues of the proton pump and identified a regulatory domain at its carboxyl terminus as well as a phosphatase domain essential to complete the catalytic cycle by hydrolysis of the phosphorylated intermediate. From the physiological point of view, he demonstrated a connection between cell growth and proton pump activity in yeast. Active nutrient transport and pH regulation were implicated in this important phenomenon. In order to extrapolate these results to animal cells, the yeast proton pump was expressed in fibroblast, which experienced elevated intracellular pH and increased proliferation (in collaboration with Rosario Perona, Madrid). In plants, the effect of auxin on cell growth was correlated with induced expression of the proton pump (in collaboration with Achim Hager, Tubingen).
Since 1990, Ramon Serrano started to work on the molecular basis of proton, potassium and sodium homeostasis, utilizing the yeast Saccharomyces cerevisiae and the plant Arabidopsis thaliana as model system. This was another aspect of the connection between membrane transport and cell growth and had the social relevance that it could generate the biotechnological tools to improve the salt tolerance of crops, an urgent need for the future of agriculture in irrigated land. This work identified the first target of sodium toxicity in living cells, the nucleotidase encoded by the yeast HAL2 gene. This enzyme is very sensitive to inhibition by Li and Na and it is required to detoxify a metabolite, adenosine-3',5'-diphosphate or PAP, which inhibits sulfate assimilation, sulfate transfer and RNA processing. Plant HAL2 homologues were characterized which could be targets of sodium toxicity. The Hal2 protein has been crystallized and the molecular basis of Li and Na inhibition characterized at the atomic level (in collaboration with Armando Albert at Madrid and Tom Blundel at Cambridge).
The transcription factor Sko1 has been identified by his laboratory as a repressor of some defense genes modulated by the yeast MAP kinase Hog1, which is activated by salt stress. This established a novel paradigm of negative control for salt stress defense genes such as ENA1 (encoding a Na+ extrussion pump) and HAL1 (encoding an activator of K+ transport). The identification of protein kinase (HAL4 and HAL5) and protein phosphatase (PPZ1) genes as modulators of K+ transport opened the molecular bases of ion homeostasis in yeast. Ppz1 is inhibited by Hal3 and the Hal3-Ppz1 interaction is pH-dependent, constituing the first pH sensors identified in cells.
Latest works of Ramón Serrano have established a connection between plant sulphydryl oxidases and ion homeostasis through regulation of potassium transport at the xylem loading interface. Also, oxidative stress has been found to desensitize ethylene responses in Arabidopsis.

Main publications last 5 years

Rausell, A., Kanhonou, R., Yenush, L., Serrano, R. and Ros, R.
The translation initiation factor eIF1A is an important determinant in the tolerance to NaCl stress in yeast and plants
Plant J. 34 (2003) 257-267

Naranjo, M.A., Romero, C., Bellés, J.M., Montesinos, C., Vicente, O. and Serrano, R.
Lithium treatment induces a hypersensitive-like response in tobacco
Planta 217 (2003) 417-424.

Mulet, J.M., Alemany, B., Ros, R., Calvete, J.J. and Serrano, R.
Expression of a plant serine O-acetyltransferase in Saccharomyces cerevisiae confers osmotic tolerance and creates an alternative pathway for cysteine biosynthesis
Yeast 21 (2004) 303-312

Mulet, J.M., Alejandro, S., Romero, C. and Serrano, R.
The trehalose pathway and intracellular glucose phosphates as modulators of potassium transport and general cation homeostasis in yeast
Yeast 21 (2004) 569-582


Gonzalez-Guzmán. M., Abia, D., Salinas, J., Serrano, R. and Rodriguez, P.L.
Two new alleles of the abscisic aldehyde oxidase 3 gene reveal its role in abscisic acid
biosynthesis in seeds
Plant Physiol. 135 (2004) 325-333

Merchan, S., Bernal, D., Serrano, R. And Yenush, L.
Response of the Saccharomyces cerevisiae Mpk1 mitogen-activated protein kinase pathway to increases in internal turgor pressure caused by loss of Ppz protein phosphatases
Eukar. Cell 3 (2004) 100-107

Portillo F, Mulet JM, Serrano R
A role for the non-phosphorylated form of yeast Snf1: tolerance to toxic
cations and activation of potassium transport.'
FEBS Lett. 579 (2005) 512-516

Forment,J., Gadea,J., Huerta,L., Abizanda,L., Agusti,J., Alamar,S., Alos,E., Andres,F.,
Beltran,J.P., Berbel,A., Blazquez,M.A1, Brumos,J., Canas,L.A., Cercos,M., Colmenero-Flores,J.M., Conesa,A, Estables,B, Gandia,M., Garcia-Martinez,J.L, Gimeno,J., Gisbert,A., Gomez,G., Gonzalez-Candelas,L., Granell,A., Guerri,J., Lafuente,M.T. Madueno,F, Marcos,J.F., Martinez,F., Martinez-Godoy,M.A., Miralles,S., Moreno,P., Navarro,L, Pallas,V, Perez-Amador,M.A., Perez-Valle,J., Pons,C., Rodrigo,I., Rodriguez,P.L., Royo,C., Serrano,R., Soler,G., Tadeo,F., Talon,M., Terol,J., Trenor,M, Vaello,L, Vicente,O, Vidal,Ch., Zacarias,L. and Conejero,V.
Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies
Plant. Mol. Biol. 57 (2005) 375-391

Yenush, L., Merchan S, Holmes J, and Serrano R.
pH-responsive, post-translational regulation of the Trk1 potassium transporter by the type I-related Ppz1 phosphatase
Mol. Cell. Biol. 25 (2005) 8683-8692

Rubio, S., Larson, T.R., Gonzalez-Guzman, M., Alejandro, S., Graham, I.A., Serrano, R.
and Rodriguez, P.L. .
An Arabidopsis mutant impaired in CoA biosynthesis is sugar-dependent for seedling establishment
Plant Physiol. 140 (2006) 830-843

Naranjo MA, Forment J, Roldan M, Serrano R, Vicente O
Overexpression of Arabidopsis thaliana LTL1, a salt-induced gene encoding
a GDSL-motif lipase, increases salt tolerance in yeast and transgenic plants.
Plant Cell Environ. 29 (2006) 1890-900

Alejandro S, Rodriguez PL, Bellés JM, Yenush L, Garcia-Sanchez MJ, Fernandez JA, Serrano R
An Arabidopsis quiescin-sulphydryl oxidase regulates cation homeostasis at the root-symplast interface
EMBO J. 26 (2007) 3203-3215


Perez-Valle J, Jenkins H, Merchan S, Montiel V, Ramos J, Sharma S, Serrano R,Yenush L.
A key role for intracellular K+ and protein kinases Sat4/Hal4 and Hal5 in the plasma membrane stabilization of yeast nutrient transporters.
Mol. Cell. Biol. 27 (2007) 5725-5736

Bueso, E., Alejandro, S., Carbonell, P., Perez-Amador, M.A., Fayos, J., Bellés, J.M., Rodriguez, P.L. and Serrano, R.
The lithium tolderance of the Arabidopsis cat2 mutant reveals a cross-talk between oxidative stress and ethylene
Plant J. 52 (2007) 1052-1065

Jian-Kang ZHU

Department of Botany and Plant Sciences. Institute for Integrative Genome Biology 2150 Batchelor Hall, University of California, USA
Riverside, CA 9252, Tel. 1 951 827 7117, Fax 1 951 827 7115
Email: jian-kang.zhu@ucr.edu

Lecture Title: Small RNAs and epigenetic regulation in plant abiotic stress responses.

 

Education
Beijing Agricultural Univ., China Soils and Agri. Chem. B.S. 1987
University of California, Riverside, CA Botany M.S. 1990
Purdue University, West Lafayette, IN Plant Physiology Ph.D. 1993

Professional Experience
2004-Present Jane Johnson Chair and Professor, Botany and Plant Sciences, University of California, Riverside
2004-2006 Director, Institute for Integrative Genome Biology, UC Riverside
2000-2003 Professor, Dept. of Plant Sciences, University of Arizona
1999 Associate Professor, Dept. of Plant Sciences, University of Arizona
1996-1998 Assistant Professor, Dept. of Plant Sciences, University of Arizona
1995 Assistant Professor, Dept. of Botany & Microbiology, Auburn University
1994 Postdoctoral Fellow, Laboratory of Plant Molecular Biology, Rockefeller University

Publications
(i) Publications most closely related to proposed project
" Jin, H., Vacic, V., Girke, T., Lonardi, S., Zhu, J.K. 2008, Small RNAs and the regulation of cis-natural antisense transcripts in Arabidopsis. BMC Mol. Biol. 9: 6
" Borsani, O., Zhu, J., Verslues, P.E., Zhu, J.K. 2005. A novel biogenesis pathway and physiological function for endogenous siRNAs from natural cis-antisense transcripts in Arabidopsis. Cell 123: 1279-1291.
" Sunkar, R., Girke, T., Zhu, J.K. 2005. Identification and characterization of endogenous small-interfering RNAs from rice. Nucleic Acids Res. 33: 4443-4454.
" Sunkar, R., Girke, T., Jain, P.K., Zhu, J.K. 2005. Cloning and Characterization of microRNAs from Rice. Plant Cell 17: 1397-1411.
" Sunkar, R., Zhu J.K. 2004. Novel and stress regulated miRNAs and other small RNAs from Arabidopsis. Plant Cell 16: 2001-2019.
(ii) Other significant publications
" Sridhar, V.V., Kapoor, A., Zhang, K., Zhu, J., Zhou, T., Hasegawa, P.M., Bressan, R.A., Zhu, J.K. 2007. Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination. Nature. 447: 735-738.
" Sunkar, R., Kapoor, A., Zhu, J.K. 2006. Posttranscriptional induction of two cu/zn superoxide dismutase genes in Arabidopsis is mediated by downregulation of mir398 and important for oxidative stress tolerance. Plant Cell 18: 2051-2065.
" Gong, Z., Morales-Ruiz, T., Ariza, R.R., Roldán-Arjona, T., David, L. and Zhu, J.-K. 2002. ROS1, a repressor of transcriptional gene silencing in Arabidopsis, encodes a DNA glycosylase/lyase. Cell 111: 803-814.
" Kapoor, A., Agarwal, M., Andreucci, A., Zheng, X., Gong, Z., Hasegawa, P.M., Bressan, R.A., Zhu, J.K. 2005. Loss-of-function mutations in a conserved replication protien suppress transcriptional gene silencing in a DNA methylation-independent manner in Arabidopsis. Current Biol. 15: 1912-1918.
" Fujii, H., Chiou, T.J., Lin, S.I., Aung, K., Zhu, J.K. 2005. A miRNA involved in phosphate-starvation response in Arabidopsis. Curr Biol. 15: 2038-2043.
" Yao Y, Guo G, Ni Z, Sunkar R, Du J, Zhu JK, Sun Q. 2007. Cloning and characterization of microRNAs from wheat (Triticum aestivum L.). Genome Biol. 8: R96

Synergistic Activities
Associate editor, Plant Physiology (2004-2005); Monitoring editor, Plant Physiology (2000-2003); Associate editor, Plant Molecular Biology (2000-present);Associate editor, Molecular, Genetics and Genomics (2005-present); Editorial board, Integrative Plant Biology (2001-); Editorial advisory board, Plant Journal (2001-); ASPB international committee, (1999-2001); International review committee and advisory board member, Institute of Bioagricultural Sciences, Academia Sinica, Taiwan (2000-); Panel member, USDA NRICGP, NSF, DOE; Conference/session organizer/chair, e.g. Gordon Conference on Temperature Stress (2005), International Workshop on Drought Tolerance, Jinan, China (2002), International Conference on Arabidopsis Research (1998; 2002, 2004), ASPP Annual Meeting (1998; 2002), NATO workshop on plant stress tolerance, Poland (1999).

Collaborators
(i) Collaborators:
John Cushman, University of Nevada, Reno
Julia Bailey-Serres, University of California, Riverside
Thomas Girke, University of California, Riverside
Pamela Ronald, University of California, Davis
Ron Mittler, Unviversity of Nevada, Reno
Martin Gollery, Unviversity of Nevada, Reno
Jeffrey Harper, Unviversity of Nevada, Reno
Michael Fromm, University of Nebraska, Lincoln
Wenyuan Song, University of Florida
(ii) Graduate and Postdoctoral Advisors:
Dr. Nam-Hai Chua (postdoctoral advisor), The Rockefeller University
Dr. Paul M. Hasegawa (Ph.D. advisor), Purdue University
(iii) Thesis Advisor and Postgraduate-Scholar Sponsor:
Dr. Deming Gong, HortResearch New Zealand
Dr. Yan Guo, China
Dr. Zhizhong Gong, China
Dr. Manabu Ishitani, Columbia
Dr. Cheol-Soo Kim, Korea
Yongsig Kim, University of Arizona
Byeongha Lee, University of Arizona
Dr. Hojoung Lee, Korea University
Dr. Jiping Liu, Cornell University
Dr. Masaru Ohta, University of Tsukuba
Dr. Huazhong Shi, Texas Tech
Dr. Liming Xiong, Danforth Plant Science Center
Dr. Monkia Mikolajczyk, The University of Arizona

Vicente RUBIO

Research Professor of the Spanish Research Council (CSIC)
Director of the Instituto de Biomedicina of Valencia (IBV-CSIC)
Instituto de Biomedicina de Valencia, Jaume Roig 11, Valencia-46010, Spain
Phone +34 96 3391772; Fax +34 96 3690800; E-mail: rubio@ibv.csic.es

Lecture Title: Arginine and Nitrogen Storage: a Structural View

Professor, Doctoral Program, Biochemistry Dept., University of Valencia
President, Spanish Society for Biochemistry and Molecular Biology (SEBBM)(09/2004-09/2008)
Head, group 39, Center for Biomedical Networked Research on Rare Diseases (CIBERER)

Education
University of Valencia Medical School M.D. (1972)
University of Valencia Medical School Ph.D. (1976) Biochemistry
Kansas University Medical Center Postdoc. (02/1974-12/1976) Biochemistry
Spanish Ministry of Education Medical Specialty (1980) Clinical Chemistry

Research and professional experience
1972-74. Fellow of the Instituto de Investigaciones Citológicas, Valencia.
1974. Spanish Ministry of Science Fellow, Dept. of Biochemistry, Kansas University Medical Center, Kansas City. Research director: Dr. Santiago Grisolia.
1975-76. Research Associate, Biochemistry Dept., Kansas University Medical Center, Kansas City.
1977-78. Staff Researcher, Cell Chemistry Dept., Instituto de Investigaciones Citológicas, Valencia.
1978-1998. Head, Cell Chemistry Dept., Inst. Investigaciones Citológicas, Valencia.
1981-1998. Research Professor, International Molecular Cytology Program, University of Kansas.
1982-87. Assistant Professor of Biochemistry, University of Valencia.
1987-present. Professor Ph.D. Programme, Dept. of Biochemistry, University of Valencia.
1987 2003. Investigador, Consejo Superior de Investigaciones Científicas (CSIC, Spanish Science Research Council).
1996-present Director, Instituto de Biomedicina de Valencia CSIC), and Head of the Molecular Enzymology and Enzymopathology Laboratory
1996-1999. Member of the Executive Board, Spanish Society of Biochemistry and Molecular Biology (SEBBM).
2002-2004 President Elect of SEBBM.
2004-present Profesor de Investigación (Research Professor) Consejo Superior de Investigaciones Científicas (CSIC, Spanish Science Research Council).
2002-2006 Professor, Severo Ochoa Molecular Medicine Chair, (CSIC-Santander Central Hispano) held each year in a Latin-American University.
2004-2008 President, Spanish Society of Biochemistry and Molecular Biology (SEBBM).

Appointments, Grants and Awards. Has been or is a member of Advisory Scientific Committees of the Fundación Valenciana de Investigaciones Biomedicas, of the Fundación de Investigación del Hospital "La Fe" (Valencia), and of Spanish granting bodies (Ministries of Health and of Education and Science), a Spanish representative to the standing Committee of the European Medical Research Council (EMRC, the medical branch of ESF), ad hoc grant reviewer for the NSF (USA) and ANEP (Spain), manuscript reviewer for several international scientific journals. Head investigator in 24 grants for research. Art/Sciences 2001 Award of the Valencian Community, and Cátedra Bernal Castejón Of the University of Zaragoza (2007).

Research interests and activity. His research is centered on studies of the structure and function of enzymes of biomedical interest, and of their complexes, having created a protein crystallography unit in Valencia. He also studies the genetic and molecular bases of inborn errors, particularly those affecting the urea cycle.
Author of over 100 research papers and 7 reviews in international journals, coauthor of 14 books, co-organizer of 10 scientific meetings, having had top responsibility or co-responsibility for the organization of 3 international meetings and two Spanishs meetings.

Ten recent publications
Llacer, J.L., Fita, I., Rubio, V. (2008) Arginine and Nitrogen Storage. Curr. Opin. Struc. Biol., in press.
Fernández-Murga, M.L., Rubio, V. (2008) The basis of the arginine sensitivity of microbial N-acetyl-L-glutamate kinases: a mutagenesis and protein engineering study with the Pseudomonas aeruginosa and Escherichia coli enzymes. J. Bacteriol. 190:3018-25
Llácer, J.L., Contreras, A., Forchhammer, K., Marco-Marin, C., Gil-Ortiz, F., Maldonado, F., Fita, I., Rubio, V. (2007) The crystal structure of the complex of PII and acetylglutamate kinase reveals how PII controls the storage of nitrogen as arginine. Proc. Natl. Acad. Sci. USA 104:17644-17649.
Marco-Marin, C., Gil-Ortiz, F., Perez-Arellano, I., Cervera, J., Fita, I., Rubio, V. (2007) A novel two-domain architecture within the amino acid kinase enzyme family revealed by the crystal structure of Escherichia coli glutamate 5-kinase. J. Mol. Biol. (2007) 367:1431-1446.
Llácer, J.L., Polo, L.M., Tavarez, S., Alarcon, B., Hilario, R., Rubio, V. (2007) The gene cluster for agmatine catabolism of Enterococcus faecalis. Studies of recombinant putrescine transcarbamylase and agmatine deiminase and a snapshot of agmatine deiminase catalyzing its reaction. J Bacteriol 189:1254-1265.
Ramón-Maiques, S., Fernández-Murga, M.L., Gil-Ortiz, F., Vagin, A., Fita, I., Rubio, V. (2006) Structural bases of feed-back control of arginine biosynthesis, revealed by the structures of two hexameric N-acetylglutamate kinases, from Thermotoga maritima and Pseudomonas aeruginosa. J. Mol. Biol. 356:695-713.
Marco-Marin, C., Gil-Ortiz, F., Rubio, V. (2005) The crystal structure of Pyrococcus furiosus UMP kinase provides insight into catalysis and regulation in microbial pyrimidine nucleotide biosynthesis. J. Mol. Biol. 352: 438-454.
Yefimenko, I., Fresquet, V., Marco-Marín, C., Rubio, V. Cervera, J. (2005) Understanding carbamoyl phosphate synthetase deficiency: impact of clinical mutations on enzyme functionality. J Mol Biol 349:127-141.
Fernández-Murga, M.L., Gil-Ortiz, F., Llácer, J.L., Rubio, V. (2004) Arginine biosynthesis in Thermotoga maritima. Characterization of the arginine-sensitive N-acetyl-L-glutamate kinase. J. Bacteriol. 186, 6142-6149.
Marco-Marín, C., Ramon-Maiques, S., Tavárez, S., Rubio, V. (2003) Site-directed mutagenesis of Escherichia coli acetylglutamate kinase and aspartokinase III probes the catalytic and substrate-binding mechanisms of these amino acid kinase family enzymes and allows three-dimensional modelling of aspartokinase.J Mol Biol 334, 459-476.

Mohammed BAAZIZ

Professor, Cadi Ayyad University, Marrakech, Morocco. former President of the Moroccan Society of Biochemistry and Molecular Biology (SMBBM) during the last period 2004-2007, Head of the Laboratory of Biochemistry and Plant Biotechnology, Faculty of Sciences-Semlalia, P.O.Box 2390, 40000 Marrakech, Morocco. E-mail:

Lecture Title: 'Peroxidases and their involvement in plant resistance to stresses. Case of date palm fusariosis (Bayoud disease)'

Scientific advisory Committee:

- 1998 until 2006: Scientific Adviser for the International Foundation for Sciences (IFS, Stockholm, Sweden).
- 1999 until 2006 : Expert for the National Commission 'Commission Nationale d'Accréditation et d'Evaluation' (CNAE),
- 1999-2000: Member of the National Commission 'Commission Nationale d'Evaluation des Programmes Thématiques d'Appui à la Recherche Scientifique' (PROTARS),

Awards

- 1999: IFS Silver Jubilee Award, by the International Foundation for Sciences (IFS, Stockholm, Sweden.

Titles
2003: Cabaliero Del Palme by San Remo city, Italy.

Reviewer

- Cahiers de la recherche, Université Hassan II-Casablanca, Maroc.
- Annals of Botany, Oxford University Press, ISSN: 0305-7364.
- Scientia Horticulturae, Elsevier ISSN: 0304-4238, depuis Mai 2007.
- The Open Horticulture Journal, Bentham Open Access ISSN: 1874-8406

Member of the editorial board:
-
'The Open Horticulture Journal', Bentham Open Access ISSN: 1874-8406

Publications for the last 5 years:

- BOUDA, S., DEL CAMPO, F., HADDIOUI, A., BAAZIZ, M. & HERNÁNDEZ, E.E. 2008. RAPD and ITS-based variability revealed in Atriplex species introduced to semi-arid zones of Morocco. Scientia Horticulturae 118, 172-179.

-MRABET, A., FERCHICHI, A., CHAIRA, N., BEN SALAH, M., BAAZIZ, M. & MRABET PENNY, T. 2008. Physico-chemical characteristics and total quality of date palm varieties grown in the southern of Tunisia. Pakistan Journal of Biological Sciences 11, 1003-1008.

- QACIF, N., BAAZIZ, M. & BENDIAB, K. 2007. Biochemical investigations on peroxidase contents of male and female inflorescences of date palm (Phoenix dactylifera L.). Scientia Horticulturae 114, 298-301.

- HADDIOUI, A. & BAAZIZ, M. 2006. Effect of salinity on seed germination and early growth of five natural populations of Atriplex halimus L. in Morocco ; Physiol. Mol. Biol. Plants 12, 247-251.

-KHALES, A. & BAAZIZ, M. 2005. Quantitative and qualitative aspects of peroxidases extracted from cladodes of Opuntia ficus indica. Scientia horticulturae 103, 209-218.

-TAHLIL, N., RADA, A., EL GHARMALI, A., BAAZIZ, M. & EL MERAY, M. 2003. Use of peroxidase activity in leaves and roots of two varieties of zucchini (Cucurbita pepo L.) as biomarkers of toxicity of soil contaminated by the spread of urban wastes. Agrochimica XLVII (3-4), 241-247.

-AZEQOUR, M., AMSSA, M. & BAAZIZ, M. 2002. Identification de la variabilité intra-clonale des vitroplants de palmier dattier issus de culture in vitro par organogenèse. Etude morphologique. C. R. Biologies 325, 947-956.

-MAJOURHAT, K., BENDIAB, K., MEDRAOUI, L. & BAAZIZ, M. 2002. Diversity of leaf peroxidases in date palm (Phoenix dactylifera L.) as revealed in an example of marginal (seedling-derived) palm groves. Scientia Horticulturae 95, 31-38.

-AZEQOUR, M., MAJOURHAT, K. & BAAZIZ, M. 2002. Morphological variations and isoenzyme polymorphism of date palm clones from in vitro culture acclimatized and established on soil in south Morocoo. Euphytica 123, 57-66.

Jacqueline C. BEDE

Assistant Professor, Department of Plant Science, McGill University, Canada
E-mail: jacqueline.bede@mcgill.ca

Lecture Title: 'Plant-Insect Interactions: the Potential Role of Cross-Talk in
Caterpillar-Mediated Circumvention of Plant Defense Responses'

Research:
Plants adjust their defense responses to target herbivores. In response, insect herbivores have evolved adaptive strategies to undermine these plant defenses. Research in Bede's laboratory lies at the boundary of plant-caterpillar interactions, characterizing the co-adaptive responses between plant and caterpillar herbivores at the molecular, biochemical and physiological levels. Understanding the underpinnings of these complex interactions is critical to gain insight into the complex molecular evolution between plants and insects and develop effective pest management strategies.

Selected publications:
Weech, MH, Chapleau, M, Pan, L, Ide, C and Bede, JC. (2008) Caterpillar saliva interferes with induced Arabidopsis thaliana defense responses via the systemic acquired resistance pathway. Journal of Experimental Botany. 59: 2437-2448

Babic, B, Poisson, A, Darwish, S, Lacasse, J, Merkx-Jacques, M, Despland, E and Bede, JC. 2008. Influence of dietary nutritional composition on caterpillar salivary enzyme activity. Journal of Insect Physiology. 54: 286-296.

Merkx-Jacques, M, Despland, E and Bede, JC. 2008. Nutrient utilization by generalist caterpillars of the beet armyworm, Spodoptera exigua. Physiological Entomology. 33: 51-61.

Darwish, S, Pan, L, Ide, C, Bede JC. 2008. Caterpillar-specific gene expression in Medicago trucatula. Plant Molecular Biology Reporter 26: 12-31.

Bede, JC, McNeil, JN, Tobe, SS. 2007. The role of neuropeptides in caterpillar nutritional ecology. Peptides 28:185-186

Bede, JC, Musser, RO, Felton, GW and Korth, KL. 2006. Caterpillar herbivory and salivary enzymes decrease transcript levels of Medicago truncatula genes encoding early enzymes in terpenoid biosynthesis. Plant Molecular Biology 60: 519-530

Merkx-Jacques, M and Bede, JC. 2005. Influence of diet on larval Spodoptera exigua glucose oxidase activity. Journal Insect Science 5: 48.

Gomez, SK, Cox, MM, Bede, JC, Inoue, K, Alborn, HT, Tumlinson, JH and Korth, KL 2005. Lepidopteran herbivory and oral factors induce transcripts encoding novel terpene synthases in Medicago truncatula. Arch. Insect Biochem. Physiol. 58:114-127.

Joaquin ARIÑO

"Catedrático" (Full Professor), Dept. Biochemistry and Molecular Biology, Autonomous University of Barcelona. Dept. Bioquímica, Fac. Veterinaria, Universitat Autònoma de Barcelona, Bellaterra, Barcelona 08193, Spain
Phone: 34-93-5812182, FAX 34-93-5812006, e-mail: Joaquin.Arino@uab.es

Lecture Title: 'Unraveling cation homoestasis in yeast: a model for plant stress adaptation'

1986. Ph.D. in Biochemistry (University of Barcelona)

Academic Positions

1986-1987. Post-doctoral Fellow (Fulbright-MEC Fellowship), University of Massachusetts Medical School.
1988-1993. "Profesor Titular" (Assistant Professor), Dept. Biochemistry and Molecular Biology, Autonomous University of Barcelona.
1993-to date. "Catedrático" (Full Professor), Dept. Biochemistry and Molecular Biology, Autonomous University of Barcelona.

Research interests

1.- Regulation of biological processes by phospho-dephosphorylation mechanisms (mainly Ser/Thr protein phosphatases)

2.- Response to stress in yeast (saline, osmotic, oxidative, alkaline).

3.- Gene regulation and genome-wide transcriptional analysis.

Publications (Last 5 years)

(Around 90 articles or reviews in international research journals. Publication listed below correspond to those appeared in the last 5 years)

- Ruiz, A., Serrano, R., & Ariño, J. (2008) Direct regulation of genes involved in glucose utilization by the calcium/calcineurin pathway. J. Biol. Chem. 283, 13923-33.

- Moreno-Romero, J., Espunya, M. C. Platara, M., Ariño, J. & Martínez M. C. (2008) A role for protein kinase CK2 in cell division in the context of Arabidopsis thaliana development: evidence using a dominant negative mutant. Plant Journal 55, 118-130.

- González, A., Larroy, C., Biosca, J.A. & Ariño, J. (2008) The use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: a note of warning. FEMS Yeast Research 8, 2-5.

- Menacho-Marquez, M., Perez-Valle, J. Ariño, J., Gadea, J. & Murguia JR (2007) Gcn2p regulates a G1/S cell cycle checkpoint in response to DNA damage. Cell Cycle 6, 2302 - 2305.

- Pagani, M.A., Casamayor, A., Serrano, R., Atrian, S. & Ariño, J. (2007) Disruption of iron homeostasis in Saccharomyces cerevisiae by high zinc levels: a genome-wide study. Mol. Microbiol. 65, 521-537.

- Aksenova, A., Muñoz, I., Volkov, K., Ariño, J. & Mironova, L. (2007) The Hal3-Ppz1 dependent regulation of nonsense suppression efficiency in yeast and its influence on manifestation of the yeast prion-like determinant [isp+]. Genes to Cells, 12 435-445.

- Pedelini, L., Marquina, M., Ariño, J., Casamayor, A., Sanz, L., Bollen, M., Sanz, P. & Garcia-Gimeno, M.A. (2007) Ypi1 and Sds22 regulate the nuclear localization and function of yeast type 1 phosphatase Glc7. J. Biol. Chem. 282, 3282-3292.

- Serrano, R., Martín, H., Casamayor, A. & Ariño, J. (2006) Signalling alkaline pH stress in the yeast Saccharomyces cerevisiae through the Wsc1 cell surface sensor and the Slt2 MAP kinase pathway. J. Biol. Chem. 281, 39785-39795.

- Platara, M., Ruiz, A., Serrano, R., Palomino, A., Moreno, F. & Ariño, J. (2006). The transcriptional response of the yeast Na+-ATPase ENA1 gene to alkaline stress involves three main signaling pathways. J. Biol. Chem. 281, 36632-36642.

- González, A., Ruiz, A., Serrano, R., Ariño, J. & Casamayor, A. (2006) Transcriptional profiling of the protein phosphatase 2C family in yeast provides insights into the unique functional roles of Ptc1. J. Biol. Chem. 281, 35057-35069.

- Ruiz, A., González, A., García-Salcedo, R., Ramos, J. & Ariño, J. (2006) Role of protein phosphatases 2C on tolerance to lithium toxicity in the yeast Saccharomyces cerevisiae. Mol. Microbiol. 62, 263-277.

- García-Salcedo, R., Casamayor, A., Ruiz, A., González, A., Prista, C., Loureiro-Dias, M.C., Ramos, J. & Ariño, J. (2006) Heterologous expression implicates a GATA factor in regulation of nitrogen metabolic genes and ion homeostasis in the halotolerant yeast Debaryomyces hansenii. Eukaryotic Cell. 5, 1388-1398.

- Ruiz, A., Ruiz, M.A., Sánchez-Garrido, M.A., Ariño, J. & Ramos, J. (2004) The Ppz protein phosphatases regulate Trk-independent potassium influx in yeast. FEBS Lett. 578, 58-62.

- Muñoz, I., Ruiz, A., Marquina, M., Barceló, A., Albert, A., & Ariño, J. (2004) Functional Characterization of the Yeast Ppz1 Phosphatase Inhibitory Subunit Hal3. A Mutagenesis Study. J. Biol. Chem. 279, 42619-42627.

- Alberola, T.M., García-Martínez, J., Antúnez, O., Viladevall, L., Barceló, A., Ariño, J. & Pérez-Ortín, J.E. (2004) A new set of DNA macrochips for the yeast Saccharomyces cerevisiae: features and uses. Intl. Microbiol. 7, 199-206.

- Bernal, D., Serrano, R., Simón, E. & Ariño, J. (2004) Copper and iron are the limiting factors for growth of the yeast S. cerevisiae in an alkaline environment. J. Biol. Chem. 279: 19698 - 19704.

- Ruiz, A., Muñoz, I., Serrano, R., González, A., Simón, E. & Ariño, J. (2004) Functional characterization of the Saccharomyces cerevisiae VHS3 gene. A regulatory subunit of the Ppz1 protein phosphatase with novel, phosphatase-unrelated functions. J. Biol. Chem. 279, 34421-34430.

- Viladevall, L., Serrano, R., Ruiz, A., Doménech, G., Giraldo, J., Barceló, A., & Ariño, J. (2004) Characterization of the calcium-mediated response to alkaline stress in Saccharomyces cerevisiae. J. Biol. Chem. 279, 43614-43624.

Reviews & Book Chapters

- Ariño, J.; Posas, F & Clotet, J. (1998) Isolation, characterization and function of novel Ser/Thr protein phosphatases in yeast. In: Methods in Molecular Biology: Protein Phosphatase Protocols. Vol 93, pp. 305 313. (J.W. Ludlow, Ed.) Humana Press, NJ.

- Pujol, G.; Ferrer, A. & Ariño, J.(1998) Protein Phosphatases 2A and protein Phosphatase X genes in Arabidopsis thaliana. In: Methods in Molecular Biology: Protein Phosphatase Protocols. Vol 93, pp. 201-212. (J.W. Ludlow, Ed.) Humana Press, NJ.

- Clotet, J. & Ariño, J. (2000) Nuevos componentes de la regulación del ciclo celular. Investigación y Ciencia. Octubre. Pp. 36-37.

- Ariño, J. (2002) Novel protein phosphatases in the yeast: an update. Eur. J. Biochem. 269, 1072-1077.

- Ariño, J. & Herrero, E. (2003) Use of tetracycline-regulatable promoters for functional analysis of protein phosphatases in yeast. Methods in Enzymology. 366, 347-358.

- Protein Phosphatases. (2004) In: Current Topics in Genetics. (J. Ariño and D. Alexander Eds.) Springer-Verlag, Berlin.

- Ariño, J., Casamayor, A., Ruiz, A., Muñoz, I., Marquina, M. (2007) The use of yeast genetic tools to define biological roles of novel protein phosphatases. In: Protein Phosphatase Protocols. Methods in Molecular Biology, vol. 365, pp. 299-308. (G. Moorhead, Ed.) Humana Press, NJ. (1-58829-711-X )

- Ruiz, A. & Ariño, J. (2007) Function and Regulation of the Saccharomyces cerevisiae ENA Sodium ATPase System. Eukaryotic Cell 6, 2175-83.

Other relevant information

- Head of the DNA Microarray and Sequencing Facility at the Autonomous University of Barcelona.
- Principal Investigator of a Research Group defined since 1994 as "High Quality Research Group" by the CIRIT (Generalitat de Catalunya).
- Coordinator of the CIRIT Network "Molecular Biology of Yeast".

For any remarks contact the Author-webmaster (M. BAAZIZ): or